Date

Lab Topics and Experiments to Begin

Pre-lab Reading in the Lab Book

Lab 1:  Wedn. Aug. 27

·      Safety

·      Microscopy

·      Exp. 4 (examine stained preps)

·      Exp. 5 (hanging-drop preparations)

·      Safety Rules on the inside cover.

·      Pages xv – xvi.

·      Pages 27-34

·      Pages 35-36

·      Pages 37-42

Lab 2: Wedn. Sept. 3 

·      Staining

·      Exp. 8 (bacterial smears)

·      Exp. 9 (simple staining)

·      Exp. 11 (gram stain)

·      Pages 55-57

·      Pages 59-63

·      Pages 65-68

·      Pages 73-78

Lab 3:  Wedn. Sept. 10

·      Exp. 12 (acid fast stain)

·      Exp. 13 (differential stain)

·      Pages 79-84

·      Pages 85-93

Lab 4:  Wedn. Sept. 17

·      Lab Protocol

·      Exp. 1 (transfer w/ aseptic technique)

·      Exp. 2 (streak for single isolates) and Environmental Sample

·      Exp. 3 (cultural characteristics)

·      Pages xvii-xviii

·      Pages 1-12

·      Pages 13-20

·      Pages 21-26

Lab 5:  Wedn. Sept. 24

·      Cultivation of Microorganisms

·      Exp. 14 (nutritional requirements)

·      Exp. 15 (differential, selective & enriched media)

·      Exp. 20 (serial dilution to quantitate viable cells)

·      Pages 95-96

·      Pages 97-102

·      Pages 103-109

·      Pages 133-138

Lab 6:  Wedn. Oct. 1

·      Exp. 16 (temperature effects)

·      Exp. 17 (pH & growth)

·      Exp. 18 (oxygen requirements)

·      Pages 111-116

·      Pages 117-120

·      Pages 121-126

Lab 7:  Wedn. Oct. 8

·      First part of lab:  Lab Exam 1.  The exam will be over the topics of labs 1-5 (not lab 6) and all associated assigned reading.

·      After the lab exam, finish experiments 16, 17 & 18

Lab 8:

Wedn. Oct. 15

·      Biochemical activities of microorganisms

·      Exp. 22 (hydrolysis of various molecules)

·      Exp. 23 (carbohydrate fermentation)

·      Exp. 24 (triple sugar-iron agar test)

·      Work with unknowns

·      Pages 147-148

·      Pages 149-154

·      Pages 155-160

·      Pages 161-164

Date

Lab Topics and Experiments to Begin

Pre-lab Reading in the Lab Book

Lab 9: 

Wedn. Oct. 22

·      Exp. 25 (IMViC)

·      Exp. 26 (hydrogen sulfide)

·      Exp. 27 (urease)

·      Exp. 29 (nitrate reductase)

·      Work with unknowns

·      Pages 165-174

·      Pages 175-178

·      Pages 179-182

·      Pages 183-188

Lab 10:  Wedn. Oct. 29

·      Exp. 30 (catalase)

·      Exp. 31 (oxidase)

·      Exp. 32 (amino acids)

·      Exp. 33 (unknowns)

·      Pages 193-196

·      Pages 197-199

·      Pages 201-206

Lab 11:  Wedn. Nov. 5 (last day to drop classes with a “W”)

·      Protozoa

·      Exp. 34 (free-living protozoa)

·      Exp. 35 (parasitic protozoa)

·      Fungi

·      Exp. 36 (molds)

·      Exp. 38 (identify unknown fungi)

·      Viruses

·      Exp. 39 (viruses: plaque forming units)

·      Page 213

·      Pages 214-220

·      Pages 221-226

·      Pages 227-228

·      Pages 229-232

·      Pages 243-247

·      Pages 249-252

·      Pages 253-255

Lab 12:  Wedn. Nov. 12

·      Physical & Chemical Agents for the Control of Microbial Growth

·      Exp. 41 (moist heat)

·      Exp. 42 (osmotic pressure)

·      Your typed report on your unknown is due by 5 pm on Friday, Nov. 14

·      Pages 265-266

·      Pages 267-273

·      Pages 274-278

Lab 13:  Wedn. Nov. 19

·      Exp. 44 (chemotherapeutic agents)

·      Exp. 46 (parts A&B, antimicrobial activity of chemical agents

·      Pages 283-288

·      Pages 297-305

Wedn. Nov. 26

No lab today.  Happy Thanksgiving Break!

Lab 14:  Wedn. Dec. 3

·      First part of lab:  Lab Exam 2 (lab exam 2 will not cover experiments 44 & 46)

·      Finish experiments 44 & 46

No lab on Wedn, Dec. 10