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Isolate #

Light = Microscopy

= ;

DNA

&= nbsp; Type

Sequenc= ing

Tow 9/21 P-2w  &n= bsp;
Site Location:    Tower Pond
Collection date: 09/21/00=
Collection:        surface
SRP:       &nbs= p;         0.01655 mg/L
NOx:       &nbs= p;         0.05327 mg/L
Conductivity:     17 μS
pH:       &nbs= p;            7.5

Notes on Tow 9/21 P-2w
7/20/01

culture started 7/8/01

Coccoid forming large masses. Does not look like a green alga.&= nbsp; Zoospores observed, but they
moved too quickly to see the flagella

18S Type C

18S

Tow 2/24 P-3w
Site Location:    Tower Pond
Collection date: 02/24/01=
Collection:        surface
SRP:       &nbs= p;         0.0835 mg/L
NOx:       &nbs= p;         0.11495 mg/L
Conductivity:     62 μS
pH:       &nbs= p;            7.01

Notes on Tow 2/24 P-3w
2/4/02

Culture started 12/21/01 (not growing well)

Eustigmatophyte. Large spherical coccoid with one or more orange bodies in cytosol.
Many refractive granules. Larger cells break easily, causing the orange body to lose its
nearly spherical shape (suggests membrane-bound?)
Pictures brightfield, with 40x and 100x objectives.

$X

Tow 2/24 P-9w
Site Location:    Tower Pond
Collection date: 02/24/01=
Collection:        surface
SRP:       &nbs= p;         0.0835 mg/L
NOx:       &nbs= p;         0.11495 mg/L
Conductivity:     62 μS
pH:       &nbs= p;            7.01

Notes on Tow 2/24 P-9w
7/1/02

Culture started 5/29/02 - grown on lab bench

Spherical coccoid with a band-shaped plastid, no pyrenoid. A pale-orange body seen in some = cells.
Reproduction not observed, but lots of old mother cell walls attached to cells. Eustig?

Tow 2/24 P-2d
Site Location:    Tower Pond
Collection date: 02/24/01=
Collection:        surface
SRP:       &nbs= p;         0.0835 mg/L
NOx:       &nbs= p;         0.011495 mg/L
Conductivity:     62 μS
pH:       &nbs= p;            7.01

Notes on Tow 2/24 P-2d
1/22/02
culture started 12/21/01 = Woods Hole medium
Eustigmatophyte with many refractile granules. Division not observed.

$X

Tow 2/24 P-6d
Site Location:    Tower Pond
Collection date: 02/24/01=
Collection:        surface
SRP:       &nbs= p;         0.0835 mg/L
NOx:       &nbs= p;         0.011495 mg/L
Conductivity:     62 μS
pH:       &nbs= p;            7.01

Notes on Tow 2/24 P-6d
1/9/02

Culture started 12/21/01 - Woods Hole medium

Spherical cells with multiple, smooth plastids. Orange body in cytoplasm. Probably a eustigmatophyte.
Reproduction not observed.

$X

18S

Tow 2/24 P-10d
Site Location:    Tower Pond
Collection date: 02/24/01=
Collection:        surface
SRP:       &nbs= p;         0.0835 mg/L
NOx:       &nbs= p;         0.11495 mg/L
Conductivity:     62 μS
pH:       &nbs= p;            7.01

Notes on Tow 2/24 P-10d
7/17/02

Culture started 5/29/02, grown up in WH

Spherical Eustigmatophyte. Very large vacuole, with plastid(s) only at one end of the cell. Yellow-orange
body present in cytoplasm. Reproduction not observed, but what appear to be remnants of mothercell
walls present near many cells.

$X

Tow 8/18 P-8d
Site Location:    Tower Pond
Collection date: 08/18/01=
Collection:        surface
SRP:       &nbs= p;         0.012 mg/L
NOx:       &nbs= p;         0.03528 mg/L
Conductivity:     26 μS
pH:       &nbs= p;            6.45

Notes on Tow 8/18P-8d
8/8/02

Culture started 6/14/02, WH

Large coccoid eustigmatophyte in clumps. Reproduction not observed. Walls of some cells have
a honeycomb pattern.

$X

Tow 8/18 T-4w =
Site Location:    Tower Pond
Collection date: 08/18/01=
Collection:        tychoplankton
SRP:       &nbs= p;         0.012 mg/L
NOx:       &nbs= p;         0.03528 mg/L
Conductivity:     26 μS
pH:       &nbs= p;            6.45

Notes on Tow8-18T-4w WH media
11/18/03
culture started 10/31/03

Eustigmatophyte

$X

18S Type O

Tow 8/18 T-8w =
Site Location:    Tower Pond
Collection date: 08/18/01=
Collection:        tychoplankton
SRP:       &nbs= p;         0.012 mg/L
NOx:       &nbs= p;         0.03528 mg/L
Conductivity:     26 μS
pH:       &nbs= p;            6.45

Notes on Tow8-18T-8w WH media
12/4/03
culture started 10/31/03

Typical coccoid eustig in clumps. Cells vary greatly in size.

$X

18S Type P

Tow 8/18 T-2d
Site Location:    Tower Pond
Collection date: 08/18/01=
Collection:    &nb= sp;   tychoplankton
SRP:       &nbs= p;         0.012 mg/L
NOx:     &nbs= p;           0.03528 mg/L
Conductivity:     26 μS
pH:      = ;            &n= bsp; 6.45

Notes on Tow 8/18 T-1d DYVI media
12/9/03
culture started 11/20/03 very slow growing

Oval eustig in clumps. Im= age with 60x.

$X

18S Type I

Tow 8/18 T-5d
Site Location:    Tower Pond
Collection date: 08/18/01=
Collection:    &nb= sp;   tychoplankton
SRP:       &nbs= p;         0.012 mg/L
NOx:     &nbs= p;           0.03528 mg/L
Conductivity:     26 μS
pH:      = ;            &n= bsp; 6.45